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Journal: Frontiers in Bioengineering and Biotechnology
Article Title: A novel method for the establishment of autologous skin cell suspensions: characterisation of cellular sub-populations, epidermal stem cell content and wound response-enhancing biological properties
doi: 10.3389/fbioe.2024.1386896
Figure Lengend Snippet: Proteomic analysis of the ACS secretome. Semiquantitative analysis using proteome arrays and quantitative detection by ELISA assays of soluble mediators secreted by ACS-derived cell populations cultured in serum-free medium at the indicated time points. Supernatants from cultures established from ACS were collected and analysed as detailed in the Methods. (A) Relative intensity values of densitometric analysis of ACS-secreted soluble factors using an angiogenesis array on days 1, 3 and 6 after isolation. (B) Relative intensity values of similar analysis using a cytokine proteome array on the same time-points post-isolation. (C) Detection of Interleukin-1 alpha, HMGB1 and Hsp90α in the ACS secretome by analyte-specific ELISA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, non-significance.
Article Snippet: Detection of analytes released in vitro from collected conditioned medium (at the indicated time points following culture initiation) from ACS-derived cultures was performed using an
Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Cell Culture, Isolation